Fig. 5

PA1-bound targets in mouse Sertoli cells. a Schematic diagram depicting the mechanism of Cut-Tag experiments in the Sertoli cells. Briefly, Sertoli cells from 20-day-old WT mice testes were collected to perform the Cut Tag experiments. Cells were sequentially incubated with anti-PA1 antibody, secondary antibody and pA-Tn5 to specifically cut the PA1-bound DNA regions and add the adapter to the end of DNA fragments. The DNA fragments were next prepared for library and sequencing. b Metaplot showing the Cut-Tag signals of PA1 (blue line) and IgG (green line) in Sertoli cells. c Heatmap of the binding sites (3553 genes) of PA1 at the positions − 3.0 kb upstream to + 3.0 kb downstream relative to transcription start site (TSS) and transcription end sites (TES). d GO-Biological process analysis of PA1 bound genes. Biological processes of positive regulation of transcription from RNA polymerase II promoter, TGF-β receptor signaling pathway, positive regulation of JUN kinase activity, cell adhesion, JUK cascade and fatty acid metabolic process were labeled with asterisks. e GO-Cellular component analysis of PA1 bound genes. Cellular components of membrane, cell junction and actin cytoskeleton were labeled with asterisks. f Venn diagrams displaying the overlap of downregulated genes and upregulated genes with PA1 bound genes. g KEGG pathway analysis of the 184 genes found in both 1045 downregulated genes in Amh-pa1^−/− Sertoli cells and PA1 bound genes. h Genomic view of PA1 (Red) and control IgG (Black) at Myo7a in wild-type Sertoli cells. Blue arrows indicated transcription start site (TSS). i Genomic view of PA1 (Red) and control IgG (Black) at Smad6 in wild-type Sertoli cells. Blue arrows indicated transcription start site (TSS)