Fig. 5

Helicase activity of SARS-CoV-2 NSP13 is required for suppression of type I IFN signaling. A Experiments in Fig. 1 were repeated with nucleic acid binding-defective mutant K345A K347A and NTP binding-defective mutant E375A of NSP13. The statistical significance for the differences between the indicated group and the empty vector control group (group 1) was determined by a one-tailed Student t test for unpaired samples with equal variance. ns: P ≥ 0.05 (not significant). **P < 0.01. ***P < 0.001. B Experiments in Fig. 3A were repeated with the indicated NSP13 mutants. Relative ratios of pSTAT1 to total STAT1, were determined by densitometry and are indicated below the blots. pSTAT1: phospho-STAT1. Results were representative of three independent experiments