Fig. 7

TSLP-induced mitophagy shifted M1/M2 chemokine expression. The M1-related chemokines A CXCL-10, B TNF-α, C IL-6, and D IL-1β in the cell culture medium of THP-1 cells and THP-1-derived M1 cells with or without TSLP treatment for 48 h were measured by ELISA. The M2-related cytokines E CCL-1 and F CCL22 in the cell culture medium of THP-1 cells and THP-1-derived M2 cells with or without TSLP treatment for 48 h were measured by ELISA. G The concentrations of CCL-22 in the culture medium of THP-1 cells and THP-1-derived M2 cells with or without pretreatment with a mitochondrial division inhibitor (mdivi-1) and 48 h of TSLP treatment were measured by ELISA. H The knockdown efficiency of PINK1 was determined by western blot. I The CCL-22 concentrations in the cell culture medium of THP-1 cells and THP-1-derived M2 cells with or without PINK1 knockdown and 48 h of TSLP treatment were measured by ELISA. Each bar represents the mean ± standard deviation of three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001