Fig. 3

Effect of activated OECs on the healing of RAEC wound scratches by co-culture method in vitro. a, b Characterization of RAECs primary cultured on day 9 under the phase contrast microscopes. Scale bar: 100 μm. c Identification of immunofluorescence for CD31 of RAECs. Scale bar: 50 μm. d Simulated wound scratches of RAECs labeled with red fluorescent probe M02 (Initial stretches column) and co-cultured with OECs from GFP transgenic rats at different time points after OEC inoculation. The final scratch areas were covered with translucent red and marked by stars. Scale bar: 100 μm. e The wound healing rate for the three groups shown in d. Scale bar: 100 μm. The data are presented as the means ± SD; n = 3 per group. *P < 0.05, **P < 0.01 vs the corresponding groups