Skip to main content
Fig. 4 | Cell & Bioscience

Fig. 4

From: Generation of immunodeficient pig with hereditary tyrosinemia type 1 and their preliminary application for humanized liver

Fig. 4

a sgRNA-targeted exon of porcine RAG1. The PAM and target sequences are colored in red and blue, respectively, and the cutting sites are indicated by small red triangles. b Appearance of the FRG model pigs. c Summary of embryo transfer data from SCNT of FRG knockout cell-line to generate mutant pig models. d Genomic PCR results confirming the pig FAH/RAG1/IL2RG knockout. e HE staining of the WT and FRG pig spleen tissue. Scale bar = 200 μm. f Flow cytometric analysis of T cells, B cells, and NK cells in the peripheral blood, bone marrow and spleen of the FRG pig.

Back to article page

Cell & Bioscience

ISSN: 2045-3701

Contact us