Table 1 List of primers used to prepare SIP mutants
SIP mutant | Mutation | Restriction site | Primers |
|---|---|---|---|
SIP K21W | K21W | BmpI | Forward: 5’-TGGTCCACTAGGAAAAGACTACG-3’ Reverse: 5’-CTCCAGCAATACTTTGACCTC-3’ |
SIP T30R_S33E | T30R_S33E | AcuI | Forward: 5’-GAAGAAAAGTCCAAGATTGAGACG-3’ Reverse: 5’-AGTAAGACGATCACGTAGTCTTTTCCTAGTG-3’ |