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Table 1 List of primers used to prepare SIP mutants

From: Siah-1-interacting protein regulates mutated huntingtin protein aggregation in Huntington’s disease models

SIP mutant Mutation Restriction site Primers
SIP K21W K21W BmpI Forward: 5’-TGGTCCACTAGGAAAAGACTACG-3’
Reverse: 5’-CTCCAGCAATACTTTGACCTC-3’
SIP T30R_S33E T30R_S33E AcuI Forward: 5’-GAAGAAAAGTCCAAGATTGAGACG-3’
Reverse: 5’-AGTAAGACGATCACGTAGTCTTTTCCTAGTG-3’