Fig. 2

NICD degradation by autophagy associates with EMT gene expression in cancer cells. A Western blot analysis. HeLa (left) and H1299 (right) cells were starved in HBSS medium for 4 h in the presence or absence of 20 μM chloroquine (CQ). After lysis, total cell lysates (30 μg) were separated by 10% or 12% SDS-PAGE and analyzed by Western blotting using primary antibodies against LC3, SQSTM1, p-ULK1-S555, p-ULK1-S757, p-AMPK-T172, AMPK, SNAI1, NICD, N-cadherin, E-cadherin, Zeb1, and Vimentin. \(\beta \)-actin was used as the loading control. B, C The level of each protein in HeLa (B) and H1299 (C) cells was quantified using NIH ImageJ software. \(\alpha \)-actin was used as the loading control. Data represent the mean (± S.D.) of three independent experiments (**P < 0.01)