Fig. 8

Identification of the interactions between ADRV proteins and host Rpb3, Rpb6, and Rpb11. A NanoLuc complementation analysis of the interactions. A significant increase (*p < 0.05) in luciferase activity was observed between Rpb3 and seven viral proteins, Rpb6 and six viral proteins, and Rpb11 and one viral protein. B Co-IP analysis of the interactions in HEK293T cells. Cells were cotransfected with plasmid pcDNA-Rpb3-3Flag and the plasmid expressing viral proteins with NlucC and HA, and coimmunoprecipitated with anti-Flag beads (left panel). The plasmid pcDNA-3Flag was used as a control (right panel). The bands corresponding to viral proteins are indicated by asterisks. C Co-IP analysis of the interactions in HEK293T cells. Cells were cotransfected with plasmid pcDNA-Rpb6-3Flag and the viral gene containing plasmids above, and coimmunoprecipitated with anti-Flag beads. D Co-IP analysis of the interactions in HEK293T cells. Cells were cotransfected with plasmids pcDNA-Rpb11-3Flag and pcDNA-29L-NlucC-HA, and coimmunoprecipitated with anti-HA beads