Fig. 3

Characterization of ADRV-85L. A Multiple amino acid sequence alignment of ADRV-85L, RGV-27R, and FV3-25R. ADRV-85L and RGV-27R have two divergent sites (shaded in black) among their 261 aa. B Prokaryotic expression and purification of recombinant ADRV-85L. The protein markers, bacteria without induction (Uninduced), bacteria with induction (Induced), and purified proteins (Purified) are labeled at the top. The recombinant proteins with molecular weights of approximately 50 kDa are indicated with asterisks. C Electrophoretic mobility shift analysis of the DNA–protein complexes. The ΦX174 DNA–protein complexes migrated more slowly with increasing protein amounts (0‒10 μg). D Temporal expression of ADRV-85L in virus infected GSTC cells by Western blot analysis. E Subcellular localization of ADRV-85L in virus infected GSTC cells by immunofluorescence. Viral nascent DNA was labeled with EdU as described above (green). ADRV-85L was detected with anti-vSSB antibody (red). Cell nuclei were stained with Hoechst 33342 (blue). An enlarged photo shows the colocalized nascent DNA, ADRV-85L, and viral factories. Visible Hoechst-labeled cytoplasmic viral factories are indicated with arrows