Fig. 8

Treatment of Naïve Recipient Human Monocytes with EXi-RVFV Activates Autophagy through the Induction of IFN-B. A Naïve U937 cells were either left untreated, or were treated with EXi-RVFV or EXu for 24 h, and whole cell lysates matched for total protein levels were subjected to western blot analysis for LC3. Mean values ± SEM for three biological replicates is shown. B Immunofluorescence microscopy analysis of LC3 expression was performed for U937 cells that were either left untreated, or were treated with EXi-RVFV or EXu for 24 h, followed by LC3 antibody staining. Green signal represents LC3 and blue signal corresponds to DAPI staining of the nuclei. C Naïve U937 cells were either left untreated, or were treated with EXi-RVFV or EXu for 24 h, and whole cell lysates matched for total protein levels were subjected to western blot analysis to measure p62 levels. Mean values ± SEM from two biological replicates is shown. D Naïve U937 cell were pre-treated with anti-IFNAR2 antibody for 4 h and subsequently were treated with EXi-RVFV for 24 h. Naïve U937 cells without anti-IFNAR1/2 antibody treatment but treated with either EXi-RVFV or EXu were also included to serve as control conditions. Western blot was performed for LC3-II analysis. Mean values ± SEM from two biological replicates are shown. *P ≤ 0.03; **P ≤ 0.01; ***P ≤ 0.005; ****P ≤ 0.0005