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Fig. 6 | Cell & Bioscience

Fig. 6

From: Antioxidant supplements promote tumor formation and growth and confer drug resistance in hepatocellular carcinoma by reducing intracellular ROS and induction of TMBIM1

Fig. 6

TMBIM1 was upregulated in HCC upon antioxidants treatment. a, b Diagrams showing number and the fold change of genes consistently activated by NAC and GSH, and upregulated in our cohort and TCGA HCC as compared to control by whole transcriptome sequencing (> 2 folds compared to control and consistent trend between NAC and GSH treatment). c TMBIM1 and CLK2 expression in NT (non tumor) and T (tumor) of HCC patients. d qPCR and western blot validation of TMBIM1 upregulation on MHCC-97L cells. e Stable knockdown of TMBIM1 in HCC cells. f ROS assay in NTC and TMBIM1 knockdown cells. g–i Sphere formation assay, cell proliferation assay, cell migration and invasion assay in TMBIM1 knockdown cells. Error bars indicate mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. NTC or control. one-way ANOVA followed by Dunnett comparison test

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Cell & Bioscience

ISSN: 2045-3701

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