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Fig. 8 | Cell & Bioscience

Fig. 8

From: From nasal to basal: single-cell sequencing of the bursa of Fabricius highlights the IBDV infection mechanism in chickens

Fig. 8

The expression of chicken Dicer/Ago/Exportin5 pathways after IBDV infection. a Results of qRT-PCR analysis of Dicer, Ago, and Exportin5. b Protein expression determined by Western blot of Dicer, Exportin5, and GAPDH. c Pie chart showing the percentage of Dicer/Drosha/Exportin5/Ago transcriptome level in each cell type. d Graph figure showing the gene transcriptome level of Dicer/Drosha/Exportin5/Ago in control and infected groups in each cell type. e t-SNE (left side) and violin plot (right side) of Dicer/Drosha/Exportin5/Ago in each cell population. Violin plots show expression in each (control and infected) sample type. The data shown are the means ± standard error. All these expressions were normalized with GAPDH mRNA expression level. These results are taken from three independent experiments. The significance level between blank and treated groups is identified by *p < 0.05, **p < 0.01, and ****p < 0.0001, determined by one-way ANOVA with Tukey’s multiple comparison test

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Cell & Bioscience

ISSN: 2045-3701

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