Fig. 7

SIRT6 silencing upregulates NF-κB and TNF-α expression in macrophage. A shControl or shSIRT6 lentivirus was transfected in RAW264.7 or BMDM for 24 h, the cells were collected and extracted for Western blotting analysis after 48 h. B, D Western blots of SIRT6, NF-κB, and TNF-α expression in shRNA transfected RAW264.7 or BMDM. C, E Quantification of SIRT6, NF-κB, and TNF-α protein levels in RAW264.7 and BMDM. The protein levels were normalized with β-actin. *P  < 0.05