Fig. 5

shSIRT6 transfection silences SIRT6 expression and inhibits phagocytosis of macrophages. A BMDM was transfected with shControl or shSIRT6 lentivirus for 24 h and cultured for further 48 h, then 1 mg/ml myelin debris was added into the cultures and the cells were collected for Western blotting after 4 h. B GFP transfection efficiency was quantitatively evaluated by fluorescence microscopy. Scale bar: 50 µm. C Western blot of SIRT6 in the blank BMDM, and shControl or shSIRT6 transfected BMDM. D Western blots of MBP, MAG and MPZ in the shControl or shSIRT6 transfected BMDM and incubated with myelin debris. E–H Quantification of SIRT6, MBP, MAG and MPZ protein levels in the blots. The protein levels were normalized with β-actin. n.s: no significance; *P  < 0.05. “−” indicates without myelin debris; “+” indicates myelin debris addition