Fig. 1

Increased expression of SIRT6 and accompanied with macrophages infiltration in the transected sciatic nerve. A Schematic diagram of macrophages infiltration in the distal of injured nerve. The black arrow points to the macrophages. B Western blots of SIRT6, F4/80, MAG, MBP, and c-Jun expression in the distal and proximal stump of the transected sciatic nerve, the intact sciatic nerve was used as the control. C–G Quantification of SIRT6, F4/80, MAG, MBP, and c-Jun protein levels in the blots. The protein levels were normalized with β-actin. n.s: non-significance; *P  < 0.05. H Immunofluorescence images showing the F4/80 positive macrophages (green) infiltrates the distal stump of the injured sciatic nerves and SIRT6 (red) expression is up-regulated in the macrophages in a time-dependent manner after transection. Scale bar:200 µm, 10 µm in the enlarged parts. I Quantification of F4/80 positive cells ratio (F4/80⁺/DAPI⁺). J Quantification of SIRT6⁺/F4/80⁺ cells ratio. *P  < 0.05. Dis distal; Prox proximal