Fig. 7

Liver-specific Gm15441 overexpression decreases Txnip protein levels and regulates glucose and lipid metabolism in mice. A Quantitative real-time PCR analysis of liver tissues from C57BL/6 wildtype mice injected with adenovirus expressing empty vector, Gm15441-L, or Gm15441-S, n = 5; B comparison of ∆Ct values between Gm15441 and Txnip (normalized to the housekeeping gene 18S) from quantitative real-time PCR analysis of liver tissues from C57BL/6 wildtype mice injected with Gm15441-L or Gm15441-S, n = 5; C Western blot analysis of liver tissues from C57BL/6 wildtype mice injected with adenovirus expressing empty vector, Gm15441-L, or Gm15441-S. Quantitation is shown in the right panel. n = 3; D 24-h fasting body weight of control, Gm15441-L or Gm15441 S overexpression mice. n = 5; E overnight fasting blood glucose levels of control, Gm15441-L or Gm15441 S overexpression mice. n = 5; F–H 24-h fasting plasma ketone body β-hydroxybutyrate (BOH) (F), plasma triglyceride (TG) (G), and liver TG (H) levels of control, Gm15441-L or Gm15441 S overexpression mice, n = 5. Error bars are SEM, *P < 0.05