Fig. 4

Gm15441 reduces exogeneous Txnip protein levels in vitro. A Sequence alignment of Txnip, Gm15441 long isoform (Gm15441-L), and Gm15441 short isoform (Gm15441-S). Gm15441-L and Gm15441-S share a common sequence (grey boxes) complimentary to Txnip CDS. Besides, Gm15441-L has a unique sequence (green box) complimentary to the Txnip 5′end. Common sequences are labelled with the same color. B Comparison of ∆Ct values between Gm15441 and Txnip (normalized to the housekeeping gene 18S) from quantitative real-time PCR analysis of mouse primary hepatocytes transduced with Gm15441-L or Txnip adenovirus for 24 h, MOI = 50; C quantitative real-time PCR analysis of mouse primary hepatocytes transduced with adenovirus expressing YFP + empty vector, YFP + Gm15441-L, Txnip + empty vector, or Txnip + Gm15441-L, for 24 h, MOI = 50; D Western blot analysis of mouse primary hepatocytes transduced with adenovirus expressing YFP + empty vector, YFP + Gm15441-L, Txnip + empty vector, or Txnip + Gm15441-L, for 24 h, MOI = 50; Quantitation is shown in the lower panel. Error bars are SEM, n = 3, *P < 0.05. N.S., not significant. E, F Quantitative real-time PCR analysis (E) and western blot analysis (F) of mouse hepatocytes AML12 transduced with adenovirus expressing Txnip + control empty vector + control siRNA (siLacZ), Txnip + Gm15441-L + siLacZ, or Txnip + Gm15441-L + Gm15441-targeted siRNA (siGm15441), for 48 h, MOI = 50; Quantitation of western blot is shown in the right panel. Error bars are SEM, n = 2, *P < 0.05