Fig. 1

Expression patterns of Gm15441 and Txnip are similar in mouse livers. A Top 5 upregulated lncRNAs in the fasting mice livers according to the genome-wide transcriptional profiling; B schematic diagram of Gm15441 and Txnip genomic organization. Lines and boxes indicate introns and exons, respectively. Exons are numbered for each gene; C quantitative real-time PCR analysis of liver tissues from C57BL/6 wildtype mice fed ad libitum, subject to a 24-h fast, or a 24-h fast followed by a 4-h refeed, n = 4; D Western blot analysis of liver tissues from C57BL/6 wildtype mice fed ad libitum, subject to a 24-h fast, or a 24-h fast followed by a 4-h refeed. Quantitation is shown in the right panel, n = 3; E quantitative real-time PCR analysis of liver tissues from ob/ob mice and their lean control mice subject to a 4-h food withdrawal, n = 4; F Western blot analysis of liver tissues from ob/ob mice and their lean control mice subject to a 4-h food withdrawal. Quantitation is shown in the right panel, n = 4; Error bars are SEM, *P < 0.05 (Fast versus Ad Libitum or ob/ob versus control), ^#P < 0.05 (Refeed versus Fast)