Fig. 5

ROS generation induced lipolysis was suppressed by N-Acetylcysteine in catalase deficient adipocytes. A Representative fluorescence images of primary white adipocytes treated as indicated and fluorescence were measured with DCFH-DA and examined under a fluorescence microscope. Scale bar represents 100 μm. B Percentage of cells showing green fluorescence (corresponding to DCFH-DA) and fluorescence intensity of adipocytes. ^##P < 0.001 WT ISO vs KO ISO; ^#P < 0.001 KO ISO vs KO ISO + NAC. C Representative fluorescence images primary white adipocytes, fixed and immunostained with anti-PLIN1 (green), ORO (red), and DAPI (blue). Scale bar represents 5 μm. D The quantification of ORO positive cells in white adipocytes from C. *P < 0.05 WT nor vs WT ISO; WT ISO vs KO ISO; KO ISO vs KO ISO + NAC. E Serum glycerol; **P < 0.01 WT nor vs WT ISO; WT ISO vs KO ISO; KO ISO vs KO ISO + NAC and F FFA level in primary white adipocytes. *P < 0.05 WT nor vs WT ISO; WT ISO vs KO ISO; KO ISO vs KO ISO + NAC. G Proteins were extracted from treated adipocytes and immunoblot analysis was performed using whole-cell lysates with the indicated antibodies. Data represent mean ± SD of three independent experiments