Fig. 4

Catalase deficiency during sustained fasting facilitates the shuttling of free fatty acid to BAT through fatty acid receptor enzymes. A Proteins were extracted from BAT of mice fasted at indicated time and immunoblot analysis was performed using whole-cell lysates with indicated antibody (n = 2). B Isolated BAT from fasted mice at indicated time were homogenized and LPL activity was measured. qPCR analysis of C CD36, D LPL and E UCP1 in the BAT of mice fasted for indicated time. F Proteins were extracted from liver of mice fasted at indicated time and immunoblot analysis was performed using whole cell lysates with indicated antibodies (n = 2). G Isolated liver from fasted mice at indicated time were homogenized and LPL activity was measured. qPCR analysis of H CD36 and I LPL genes in liver of mice fasted for indicated time. Values represent mean ± SD (n = 3, 4). *P < 0.05 WT 48 h fasting versus KO 48 h of fasting; ^##P < 0.001 WT 48 h fasting versus KO 48 h of fasting