Fig. 2

Catalase deficiency during sustained fasting decreases the fat mass that augmented lipolysis in WAT. A Images of subcutaneous and gonadal (sWAT, gWAT) white adipose tissuefrom mice (n = 6). B Analytical weight of gWAT and sWAT in gram (g) C Representative H&E staining of WAT from mice. D Representative fluorescence images of WAT, fixed and immunostained with anti-PLIN1 (green) and DAPI (blue).Scale bar represents 20 μm. E FFA and F glycerol level from mice serum fasted for indicated time (n = 6). G Proteins were extracted from WAT and immunoblot analysis was performed using whole-cell lysates with the indicated antibodies (n = 3). H Total ROS was measured from WAT. Values represent mean ± SD (n = 3, 4). *P < 0.05 WT 48 h fasting versus KO 48 h of fasting