Fig. 2
Role of AR activity in LNCaP cell migration. A Gene expression of AR in therapy-induced developmental reprogrammed-PCa cells (n = 3) as compared to parental PCa cell lines (GSE66852). Data were shown as box and whisker plots. Box, interquartile range (IQR); whiskers, 5–95 percentiles; and horizontal line within the box, median. B AR-mediated inhibition of LNCaP cell migration. LNCaP cells were treated with flutamide (AR antagonist, 1 μM) in the presence or absence of dihydrotestosterone (DHT, AR agonist, 2 nM) for 24 h. Migratory cell numbers were determined by the IncuCyte ZOOM live-cell analysis system. Data represent means ± SD (n = 4, ***P < 0.005 vs vehicle-treated control, #P < 0.05 vs flutamide-treated group). C Development of AR knockout LNCaP cells by CRISPR/Cas9 system. Expression of AR was determined by immunoblot analysis. D Migration of AR knockout LNCaP cells. Migratory cell numbers were determined by IncuCyte ZOOM live-cell analysis system for 24 h. Data represent means ± SD (n = 4, *P < 0.05, **P < 0.01 vs vehicle-treated control)