Fig. 3

Relative gene expression during osteogenesis and adipogenesis. A Adipogenic differentiation of BM-MSCs characterized by increased expression of peroxysome proliferator-activated receptor (PPAR)-γ, Fatty Acid Binding Protein (FABP4) and lipoprotein lipase (LPL) and by staining of lipid droplets by Red Oil O in differentiation versus proliferative conditions at day 21. B Relative expression of RGCC, TSPAN2 and NMB at different time points during adipogenic differentiation (black line) or in proliferative conditions (hatched line). C Osteogenic differentiation of BM-MSCs characterized by increased expression of RUNX2, osteocalcin (BGLAP), alkaline phosphatase (ALPL) and by Alizarin Red S staining in differentiation versus proliferative conditions at day 21. D Relative expression of RGCC, TSPAN2 and NMB at different time points during osteogenic differentiation (black line) or in proliferative conditions (hatched line). Results are expressed as mean fold change ± sem and statistical significance was indicated as *: p ≤ 0.05, **: p ≤ 0.01, ***: p ≤ 0.001 when samples were compared to day 0 and as ^$: p ≤ 0.05, ^$$: p ≤ 0.01 when proliferation and differentiation conditions were compared (n = 6 biological replicates)