Fig. 8

RNF144A-AS1 was a TGF-β- and hypoxia-inducible gene in GC. A Heat map presentation of the results generated by GSEA analysis based on the expression of LOX and RNF144A-AS1 from the CCLE database. The red shades represent high NES scores, and the blue shades represent low NES scores. B–D qRT-PCR analysis of RNF144A-AS1 (B), miR-30c-2-3p (C), and LOX (D) expression under hypoxic conditions. E, F Pearson correlation analysis of RNF144A-AS1 (E) and LOX (F) with HIF-1α in 375 GC tissues from the TCGA database. G Expression level of RNF144A-AS1, miR-30c-2-3p, and LOX in HIF-1α-knockdown group. H, I Pearson correlation analysis of RNF144A-AS1 (H) and LOX (I) with TGF-β1. J Expression level of RNF144A-AS1, miR-30c-2-3p, and LOX in GC cells treated with recombinant human TGF-β1 or not. Error bars, mean ± SD from triplicate samples. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t‐test unless otherwise specified