Fig. 6

Propranolol treatment inhibited the IFN-I-mediated induction of antiviral genes and counteracted IFN-mediated inhibition of viral propagation in HGC-27 human gastric cancer cells. A and B Dose effects of propranolol were evaluated on the IFN-induced inhibition of T1012G production in HGC-27 cells. Cells were treated with 300 ng/well of human recombinant IFN-α/β and different concentrations (0, 10, 20, or 40 μmol/l) of propranolol for 48 h and infected withT1012G (0.01 MOI). Two days after infection, the cells and medium were harvested, and yields of virus were determined were determined on Vero cells. C and E HGC-27 cells were incubated with 40 μmol/l propranolol (for 48 h) and/or 300 ng/well human recombinant IFN-α/β (for 6 h) and then harvested for the assessment of protein expression of STAT3, p-STAT3, PKR, and p-PKR by western blot analysis. D and F Quantification of C, E. Results are presented as mean ± SEM, significant differences were evaluated using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001(Dunnett’s test for multiple comparisons)