Fig. 5
LCN2 promotes inflammation following Mtb infection. WT and LCN2−/− mice were intratracheally infected with Mtb (106 CFU) for 10 days. A Lung inflammation was determined by hematoxylin and eosin staining. Sale bars = 200 μm. B Levels of inflammatory cytokines in sera were determined using a CBA mouse inflammation kit by flowcytometry. Data are shown as mean ± SD (n = 9, 3 mice/group) and are pooled from three independent experiments. C Bacterial loads in the lungs were analyzed by CFU assays on 7H10 plates. Data are shown as mean ± SD (n = 9, 3 mice/group) and are are pooled from three independent experiments. Statistically significant differences were determined by Mann–Whitney test (non-parametric unpaired two-tailed t-test). *p < 0.05, **p < 0.01, and ***p < 0.001