Fig. 1
From: The mechanism of Annexin A1 to modulate TRPV1 and nociception in dorsal root ganglion neurons
Generating of AnxA1−/− mice and detecting nociceptive sensation. a Gene targeting strategy. Two loxP sites were inserted on both sides of exon 6 of the AnxA1 locus followed by a FRT-flanked NEO cassette (F and NEO). To selectively delete AnxA1 gene in DRG neurons, we crossed AnxA1loxP/+ mice with Avilcre mice to eventually obtain Avilcre-AnxA1loxP/loxP (AnxA1−/−) mice and littermate controls (Avilcre-AnxA1+/+, Con.). b DNA band image of AnxA1 in control and AnxA1−/− mice from PCR genotyping results. c The gross physical appearance and the body weight gain in control and AnxA1−/− mice. d Immunofluorescent staining of DRG frozen sections isolated from control and AnxA1−/− mice stained with antibody to ANXA1. Scale bar, 100 µm. Measurement of thermal (e–g) and mechanical (h and i) nociception between control and AnxA1−/− mice. e Quantification of the thermal latency to radiant heat. f Quantification of the thermal latency to hot plate at 50 ℃ (AnxA1−/− versus control group, ***P < 0.001, Student’s t-test). g Quantification of the response latency to cold plate at 4 ℃. h Quantification of the threshold to von Frey filaments. i Quantitative analysis of mechanical pressure force in Randall-Selitto test (AnxA1−/− versus control group, P > 0.05, Student’s t-test). Measurement of chemical nociception (j and k) between control and AnxA1−/− mice. j Quantitative analysis of the licking or biting duration over 10 min after unilateral injection of 40 μg menthol, 5% mustard oil or 25 μg capsaicin into the hindpaw of mice (AnxA1−/− versus control, **P < 0.01, Student’s t test). k Quantitative analysis of the licking or biting duration over 60 min after injection of 1% formalin into the hindpaw of mice (AnxA1−/− versus control, *P < 0.05, **P < 0.01, Student’s t-test). Quantitative analysis of the withdrawal latency to radiant heat in Hargreaves test (l) and threshold in von Frey test (m) after unilateral injection of CFA into the hindpaw of mice (AnxA1−/− versus control, **P < 0.01, ***P < 0.001, two-way ANOVA followed by Sidak’s multiple comparisons test, n = 10 in control group, n = 8 in AnxA1−/− group). All data are represented as mean ± SD