Fig. 5

C1GALT1 is transcriptionally activated by SP1 in GC. a Scatter diagrams for C1GALT1 expression versus SP1 expression in GC samples based on the TCGA and GTEx databases(Spearman’s rank correlation test). b Representative images and quantitative analysis of SP1 expression in GC tissues (Spearman’s rank correlation test). Scale bars, 100 μm. c Western blot analysis of SP1 expression in different GC cells. d The effect of SP1 on C1GALT1 expression was detected by Western blot. e Schematic presentation of SP1 binding sites within the promoter region of C1GALT1. f The binding of SP1 to the C1GALT1 promoter was analyzed by ChIP-PCR. g, h Relative luciferase activity was examined by the dual-luciferase reporter assay. i–k Cell proliferation, migration, and invasion were assessed by CCK-8, Transwell migration, and Matrigel invasion assays. siNC: cells transfected with negative control siRNA; si1: cells transfected with SP1 siRNA1; si2, cells transfected with SP1 siRNA2; Ctrl, cells transfected with control plasmid; SP1, cells transfected with SP1 plasmid; OV: cells transfected with C1GALT1 plasmid; WT: Wild-type; MUT: mutant. *p < 0.05, **p < 0.01 compared with the siNC or Ctrl group (Student’s t-test or one-way ANOVA)