Fig. 6

E2F1 promoted SNHG1 transcription. a TCGA data showed that E2F1 is positively correlated with SNHG1 in pan-caner and in breast cancer. Knockdown efficacy of siE2F1 at mRNA (b) and protein (c) levels in MDA-MB-468, Hs578T, and HEK293T cells. d Knockdown of E2F1 downregulated SNHG1 expression. e Knockdown of E2F1 downregulated TERT mRNA expression. f Knockdown of E2F1 upregulated miR-18b-5p expression. g JASPAR predicted that E2F1 could bind with the promoter of SNHG1. h Schematic representation of the predicted E2F1 binding sites within the promoter of SNHG1. i Chromatin immunoprecipitation assay showed the binding of E2F1 and SNHG1 promoter region in Hs578T and HEK293T cells. j, k Silencing TERT could downregulated the expression of SNHG1 and upregulated the expression of miR-18b-5p. l Working model of the study. Data are presented as the mean value from three independent experiments ± S.D. *P < 0.05; **P < 0.01; ***P < 0.001; *****P < 0.0001; NS no significance