Fig. 2
Time-dependent expression of Adamts-1 induced by E2 in the mouse uterus. a, b Spatiotemporal expression patterns of Adamts-1 and Egr1 in the uterus at several time points after E2 treatment. RT-PCR and real-time RT-PCR a and Western blot analyses b (4–5 mice in each group). Error bars represent standard deviation. *P < 0.05, **P < 0.01. c Immunofluorescence staining of ADAMTS-1 showed that ADAMTS-1 is specifically localized in the uterine epithelium (white arrowheads). The second panels (i to iv) show the enlarged images of the boxed areas in the first panels. Three independent fields were analyzed for each mouse (2–3 mice in each group). Green, blue, and red colors indicate ADAMTS-1, E-cadherin, and nucleus, respectively. GE, glandular epithelial cells; LE, luminal epithelial cells; S, stromal cells. Scale Bar: 2 µm. RT-PCR and real-time RT-PCR were performed to analyze the relative levels of Adamts-1 induced in the uteri of OVX mice by P4 alone d or by a combination of E2 + P4 e at different time points post hormone treatment (4–5 mice for each time point). Hoxa10 and Cox1 represent temporal expression markers for the time-dependent hormonal response. rPL7 represents the loading control