Fig. 1

Adamts-1 expression is significantly reduced in the uteri of Egr1(−/−) mice. a RT-PCR and real-time RT-PCR analyses of the relative amounts of Adamts-1 and Egr1 mRNAs in the uterus (4–5 mice in each group). b Western blot analyses of ADAMTS-1 and EGR1 expression (3–4 mice in each group). Error bars represent standard deviation. *P < 0.05. c Immunofluorescence staining of ADAMTS-1 showed that its expression was significantly reduced in the uteri of Egr1(−/−) mice. The second panels (i and ii) show the enlarged images of the boxed areas in the first panels. White arrowheads indicate ADAMTS-1 positive cells. Three independent fields were analyzed for each mouse (2–3 mice in each group). Green, blue, and red colors indicate ADAMTS-1, E-cadherin, and nucleus, respectively. GE glandular epithelial cells, LE luminal epithelial cells, S stromal cells. Scale Bar: 25 µm. d RT-PCR and real-time RT-PCR analyses of Adamts-1 mRNAs in the isolated uterine stromal cells (SC) and epithelial cells (EC). Keratin and Desmin are EC and SC marker genes, respectively (six mice in each group). *P < 0.05