Fig. 3

Expressions of P-Stat3 and CNTF in the retina of each group. A Time course of the treatment for each group of animals. WB, western blotting; ON, optical nerve. B P-Stat3 leve and CNTF expressions 21 days after intravitreal injection in four groups. C P-Stat3 level and CNTF expressions 28 days after intravitreal injection in AAV shRNA + ONI and AAV PirB shRNA + ONI groups, D P-Stat3 level and CNTF expressions 35 days after intravitreal injection in AAV shRNA + ONI and AAV PirB shRNA + ONI groups. E P-Stat3 level and CNTF expressions 49 days after intravitreal injection in AAV shRNA + ONI and AAV PirB shRNA + ONI groups. GAPDH and Stat3 were used as internal references. Original blots are shown in c, d, and e. The ratios of gray values of target proteins to those of respective internal references are provided. The experiment was repeated three times (n = 4). Data are presented as the mean ± standard deviation. One-way ANOVA was performed, and Student’s t-test was used for group-pair comparisons. *p < 0.05 and **p < 0.01