Fig. 2

The effect of PirB knockdown in the retina by AAV PirB shRNA. A GFP (green, 488) expression in the retina transfected with AAV PirB shRNA or AAV shRNA for 21 days. red * represents the injection site, and the purple dotted line shows the area without the retinal tissue. Scale bars: 150 μm. B GFP staining in the PBS group. DAPI represents nuclear staining (blue fluorescence). Scale bar: 250 μm. C GFP (green, 488) and β-Ш Tubulin (red, 594) fluorescent staining in the AAV PirB shRNA group. DAPI represents nuclear staining (blue). Scale bar: 250 μm. Small white arrows in c show GFP and β-Ш Tubulin co-localization. D GFP (green, 488) and Vimentin (red, 594) fluorescent staining in the AAV PirB shRNA group. DAPI represents nuclear staining (blue). Scale bar: 250 μm. Small white arrows in d show GFP and Vimentin co-localization. E Bar charts showing PirB expression in the retina tissue. GAPDH was used as an internal reference. The ratios of gray values of target proteins to those of respective internal references are provided. The experiment was repeated three times (n = 4). Data represent the mean ± standard deviation. One-way ANOVA was performed, and Student’s t-test was used for group-pair comparisons used. **, p < 0.01