Fig. 5
MDLS promoted teratoma formation of P19 cells and enhanced the differentiation potential of P19 cells. The P19 cells were treated with MDLS or DMSO for 7 days. Subsequently, the P19 cells were harvested at a density of 5 × 107 cells/mL. Then, 200 μl of the cell suspension was injected subcutaneously into nude mice. After 3 weeks, the mice were euthanized by cervical dislocation, and the teratomas were resected and measured using calipers. A Phenotype of nude mice after 3 weeks of treatment. B Appearance of teratomas derived from MDLS-treated and DMSO-treated cells. C The volume of teratomas monitored at the end of the experiment. D The weight of teratomas monitored at the end of the experiment. E The paraffin sections from the teratoma tissue specimens were stained with hematoxylin and eosin, and these sections were observed under a light microscope. F Top: the expression levels of class III β-tubulin, cTnT and AFP in the teratomas of P19 cells were analyzed using Western blot analysis. Equal protein loading was evaluated using GAPDH. P < 0.05, compared with the DMSO-treated group. Bottom: quantitative analysis of Western blotting data by image J soft-ware. Levels of these proteins were normalized to the corresponding GAPDH band expressed as relative fold changes in comparison to control samples. Values correspond to the mean ± S.E. of three independent experiments