Fig. 2

EHMT2 is required for the tumorigenesis of HCC. A The EHMT2 protein level in Hep3B and Huh1 cells was determined by Western blot. The red labels indicate the EHMT2 double knockout (EHMT2^−/−) single clones. n = 3. B Cell proliferation was determined by Ki67 staining. # indicates EHMT2^−/− single clones in 2A. Scale bar, 100 μm. n = 6 (mean ± SD); **p < 0.01 and ***p < 0.001 by student's t-test. C MTT assay determined the cell growth curve of Hep3B and Huh1 cells. EHMT2^−/− single clones were the same as 2A. n = 6 (mean ± SD); *p < 0.05, **p < 0.01 and ***p < 0.001 by two-way ANOVA followed by post hoc Bonferroni multiple comparisons. D Soft agar assay determined the anchorage-independent growth ability of Hep3B and Huh1 cell lines. EHMT2^−/− single clones were the same as 2A. The spheres with a diameter > 25 μm were considered tumorspheres. n = 6 (mean ± SD); ***p < 0.001 by one-way ANOVA. E. Final tumor weight of Hep3B and Huh1 in the xenograft assay. Three EHMT2^−/− single clones of Hep3B and Huh1 in 2A were pooled together for xenograft assay. n = 6 (mean ± SD); ***p < 0.001 by student's t-test