Fig. 5
From: TRIM28 inhibits alternative lengthening of telomere phenotypes by protecting SETDB1 from degradation
TRIM28 promotes H3K9me3 occupancy on telomere in ALT cells. A IF assay of TRIM28-deficient U2OS cells was performed using antibodies against H3K9me3. B Telomere ChIP analysis in U2OS cells with overexpressed TRIM28 or vector were performed using the indicated antibodies. Rabbit IgG served as a negative control. The precipitated DNA was slot blotted and probed with biotin-labeled telomere and Alu probes. C Relative signals from (B) were quantified and normalized to histone H3 signals. *P < 0.05, **P < 0.01, Student’s t-test