Fig. 3
From: TRIM28 inhibits alternative lengthening of telomere phenotypes by protecting SETDB1 from degradation
TRIM28 knockdown decreases the C-circle level of U2OS cells. A U2OS Genomic DNA (25, 50, or 100 ng) from shLuci and shTRIM28 cells were used for the C-circle assay. An Alu repeat probe served as internal control. B Quantification histogram of data from (A). Intensity values from TRIM28 knockdown cells were normalized to the control cells. Error bars indicate standard error (n = 3). ***P < 0.001, Student’s t-test. C C-circle formation assays via ∅29 amplification and slot blotting in VA13 cells. D Overexpression of TRIM28 in U2OS cells was detected with TRIM28 antibodies. E C-circle formation assays via ∅29 amplification and slot blotting in TRIM28 overexpressed U2OS cells