Fig. 2

TRIM28 knockdown induces telomere dysfunction-induced foci. Knocking-down efficiency of shRNAs targeting TRIM28 were detected by Q-PCR (A) and by western blot (B). *P < 0.05, **P < 0.01, Student’s t-test. C The effect of TRIM28 deletion on the growth of U2OS cells was detected by cell counting. The initial cell number was 50,000. **P < 0.01, ***P < 0.001, Student’s t-test. D U2OS cells infected with viruses of control (shLuci) or two different shRNAs against TRIM28 were stained by IF-FISH assay with a telomere probe (green) and anti-53BP1 antibodies (red). DAPI was used to show the nuclei. Arrows indicate colocalization signals. E The data of (D) was quantified and plotted as a histogram, about 200 cells were analyzed for each line and those with ≥ 5 53BP1-telomere co-localized foci were counted as positive. Error bars represent SD (n = 3). ***P < 0.001, Student’s t-test