Fig. 5

YTHDF2 alleviating myocardial hypertrophy dependents on interacting with Myh7 mRNA. A Western blotting analysis of YTHDF2 and its mutant expression in the primary cardiomyocytes infected with the indicated FLAG-tagged AAV vectors. B Microscopic images of the CMs. Cells were seeded in 24-well plates, infected with the indicated FLAG-tagged AAV vectors for 48 h, then treated with ISO, PHE or not for 48 h. Both cell groups were then stained with antibodies directed against α-actinin (Green) and with DAPI (Blue) for nuclear staining. C Cell size was measured in 10 fields/well in all groups (n = 3 independent experiments; n > 50 cells per experimental group; *p < 0.05). D RIP analysis of the interaction between YTHDF2 and ANP/BNP/Myh7/Collagen type 1 (col 1) mRNA in the cardiomyocytes stimulated with ISO, PHE or DMSO. E Western blotting analysis of MYH7 and YTHDF2 expression in the primary cardiomyocytes transfected with si-nc or si-Myh7. F Microscopic images of the CMs. Cells were seeded in 24-well plates, transfected with si-nc or si-Myh7 and AAV9-FLAG-YTHDF2 vector for 48 h, then treated with ISO or PHE for 48 h. Both cell groups were then stained with antibodies directed against α-actinin (Green) and with DAPI (Blue) for nuclear staining. G Cell size was measured in 10 fields/well in all groups. (n = 3 independent experiments; n > 50 cells per experimental group; *p < 0.05). H RIP analysis of YTHDF2 or YTH-del binding on the Myh7 mRNA (n = 3 independent experiments, *p < 0.05)