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Table 1 Key reagents and resources

From: PIAS1 potentiates the anti-EBV activity of SAMHD1 through SUMOylation

Reagent or resource Source Identifier
Antibodies and reagents
 Anti-phospho-SAMHD1-T592 Cell Signaling Tech Cat# 15,038
 Anti-SAMHD1 Bethyl Cat# A311-354
 Anti-HA Roche Cat# 11–867-431–001
 Anti-HA-HRP Cell Signaling Tech Cat# 14,031
 SUMO2 Conjugation Kit UBPBio Cat# J3120
 SUMOlink SUMO2/3 Kit Active motif Cat# 40,220 (discontinued)
 Anti-V5 magnetic beads MBL Cat# M167-11
 Anti-SUMO2/3 MBL Cat# M114-3
 Anti-SUMO2/3 Active motif Cat# 101,898
 Anti-V5-HRP Thermo Fisher Cat# R961-25
 Anit-V5 Thermo Fisher Cat# R960-25
 Anti-p53 Active motif Cat# 100,853
 Mouse anti-β-actin antibody MP Biomedicals Cat# 691,001
 Anti-human IgG (for IgG cross-linking) MP Biomedicals Cat# 55,087
 Anti-ZTA(BZ1) Santa Cruz Cat# sc-53904
 Rabbit IgG Cell Signaling Tech Cat# 2729
 Halo-tag protein purification kit VWR/Promega Cat# PAG6790
 Glutathione Sepharose 4B GE Healthcare Cat# 17–0756-01
 Factor Xa NEB Cat# P8010
 ChIP-Enzymatic Chromatin IP Kit Cell Signaling Tech Cat# 9003
Constructs
 pLX304-SAMHD1 (with PAM mutated) Li Lab [6] pKZ175
 pLX304-SAMHD1-K469R/K595R/K622R (with PAM mutated) This study pSF029
 pLX304 Addgene Plasmid# 25,890
 LentiCRISPR v2 vector Addgene plasmid# 52,961
 pMD2.G Addgene plasmid# 12,259
 psPAX2 Addgene plasmid#12,260
 pSG5-ZTA Hayward Lab Collection NA
 Halo-HA-BGLF4 Hayward Lab Collection pGL772
 Halo-HA-UL97 Hayward Lab Collection pGL798
 Halo-V5-PIAS1 Li Lab [31] pKZ28
 pCMV-XL4-SAMHD1 Origene Cat# SC114650
 pCMV-XL4-SAMHD1-K469R This study pSF004
 pCMV-XL4-SAMHD1-K595R This study pSF005
 pCMV-XL4-SAMHD1-K622R This study pSF006
 pGEX-5x-2-SAMHD1 Li lab [6] pKZ49
 pGEX-5x-2-SAMHD1-K469R This study pSF017
 pGEX-5x-2-SAMHD1-K595R This study pSF018
  pGEX-5x-2-SAMHD1-K622R This study pSF019
pGEX-5x-2-SAMHD1-K469R/K595R/K622R This study pSF028
Cell lines
 Akata (EBV +) Hayward Lab Collection NA
 Akata (EBV +)-PIAS1-KO Li Lab [31] NA
 Akata (EBV +)-SAMHD1-sg1 Li Lab [6] NA
 Akata (EBV +)-SAMHD1-sg1-pLX-SAMHD1 This study NA
 Akata (EBV +)-SAMHD1-sg1-pLX-SAMHD1- K469R/K595R/K622R This study NA
 Akata (EBV +)-SAMHD1-sg1-pLX-Vector Li lab [6] NA
 293 T cells Hayward Lab Collection NA
 HEK 293 (EBV +) [50] NA