Fig. 5

Synergistic effects of TPD52 knockdown and inhibition of HIF in tumor-xenograft mouse models. a The experimental schema. TPD52-knockdown or control SAS cells were xenografted in a unilateral flank of mice (open triangle). After 1 w of maintenance, this day was designated as “0 d.” Saline or 0.5 μg/kg bodyweight of PX-478 were injected intraperitoneally, and bodyweight and tumor volumes were measured every three days (arrow). On day 18, the mice were euthanized, and the tumor was collected (closed triangle). b Average body weight changes observed in the mice. c Average tumor volume changes in the mice. *, p < 0.05 versus control. d, e and f Photographic images of tumor-xenografted mice and collected tumors on day 18. Experiments were repeated 3 times