Fig. 2
Effect of knockdown of HIF-1/2α and induction of HIF. a siRNAs for HIF-1α or 2α, or control siRNA were transfected into SAS cells, and incubated under normoxia conditions for 24 h. Then, the cells were exposed to hypoxia for 0, 8, 16, and 24 h, and were subjected to RT-qPCR and reporter assay to determine TPD52. The value at time 0 is designated as “1,” and relative values are shown. b SAS cells were exposed to normoxia for 0, 8, 16, and 24 h, in the presence or absence of 10 μM CoCl2. Then, the cells were subjected to RT-qPCR and reporter assays to detect TPD52. The value at time 0 is designated as “1,” and relative values are shown. c siRNAs for HIF-1α or 2α, or control siRNA were transfected into SAS cells, and the cells were exposed to hypoxia for 0, 8, 16, and 24 h. Then, the cells were subjected to western blotting analysis to detect TPD52, HIF-1α, HIF-2α, and β-actin. d SAS cells were exposed to normoxia for 0, 8, 16, and 24 h, in the presence or absence of 10 μM CoCl2. Then, the cells were subjected to western blotting analysis to detect TPD52, HIF-1α, HIF-2α, and β-actin. Experiments were repeated 3 times