Fig. 6
Intestinal epithelium-specific deletion of PRMT1 reduces Paneth cells in the crypt. A PRMT1 protein is low or absent in Paneth cells in the adult wild type intestine. The small intestine of 8-week-old adult wild type (PRMT1fl/flVil-Cre−/−) or PRMT1 knockout (PRMT1fl/flVil-Cre+/−) mice were sectioned and stained with anti-PRMT1 antibody for PRMT1 expression (red) or anti-lysozyme antibody for Paneth cells in the crypt (green). Note that the signals for PRMT1 and lysozyme were in different cells in the wild type intestine, with PRMT1-expressing cells surrounding the lysozyme-positive Paneth cells, which is consistent with PRMT1-expressing cells being the crypt base stem cells). Expectedly, PRMT1 was not detected in the knockout epithelium. B, C PRMT1 knockout reduces Paneth cells in both proximal and distal small intestine. The proximal and distal small intestine of wild type and PRMT1 knockout mice were stained with anti-lysozyme antibody for Paneth cells and Hoechst for DNA (B) and the number of Paneth cells were quantified from multiple sections per animal (C). For both PRMT1 knockout and control wild type littermates, n = 4. ***p < 0.001. ns no significant