Deletion of ER-retention motif on SARS-CoV-2 spike protein reduces cell hybrid during cell–cell fusion

Cell & Bioscience

Table 2 Hybrid or syncytia formation in co-culture combinations of transduced cell lines

Effector cells	Target cells	hybrid	Effector cells	Taget cells	Syncytia
S WT-GFP-293T	hACE2-mCherry—293T	+ + + +	S D19-GFP-293T	hACE2-mCherry—293T	+ + +
S WT-GFP-293T	hACE2-mCherry—A549	+ +	S D19-GFP-293T	hACE2-mCherry—A549	+ +
S WT-GFP-A549	hACE2-mCherry—293T	+ + + +	S D19-GFP-A549	hACE2-mCherry—293T	+ + +
S WT-GFP-A549	hACE2-mCherry—A549	±	S D19-GFP-A549	hACE2-mCherry—A549	±
S WT-GFP-A549	hACE2-mCherry—K562	+	S D19-GFP-A549	hACE2-mCherry—K562	+ +
S WT-GFP-K562	hACE2-mCherry—A549	+ +	S D19-GFP -K562	hACE2-mCherry—A549	+ +
S WT-GFP-K562	hACE2-mCherry—K562	+	S D19-GFP-K562	hACE2-mCherry—K562	+
S WT-GFP-K562	hACE2-mCherry-SKHep1	+ +	S D19-GFP-K562	hACE2-mCherry-SKHep1	+ +

The size of fused cells less than 20 uM defined as “ ± ”; 20–40 uM as “ + ”, 40–80 uM as “ + + ”, 80–120 uM as “ + + + ”, larger than 120 uM as “ + + + + ”

ISSN: 2045-3701