Fig. 3

Representative images of A549 cells transduced with S-WT-EGFP, S-Δ19-EGFP and hACE2-mCherry vectors. Top panels show transduced A549 cells under EVOS FL color image systems, Scale bar equals 400 µm. Bottom two rows show successful expression of S-WT-EGFP, S Δ19-EGFP, or hACE2-mCherry in A549 cells by high resolution confocal microscopy. Middle row represents DIC images. Bottom row represents merged confocal images (DAPI, blue; S-WT-EGFP or S-Δ19-EGFP, green; hACE2-mCherry, red). Scale bar equals 40 µm