Fig. 5

Rev-erbα regulated lipid metabolism by enhancing autophagy activity in vivo and vitro. A Electron microscopy of liver tissues of EtOH fed mice injected with or without SR8278 at 10 μm and at 2 μm (N nucleus, M mitochondria, LD lipid droplet; triangle refers to autophagy; arrow refers to lysosome) (n ≥ 6). B Histopathological analysis of P62 and Lc3 by immunohistochemistry in the liver of EtOH-fed mice injected with or without SR8278 (scale bar = 100 μm or 20 μm). Bar represents the mean ± SEM. Significance *P < 0.05, **P < 0.01 vs. CD-fed group. ^#P < 0.05, ^##P < 0.01 vs. EtOH-fed group. C Lysosome staining with Lyso Tracter Green DND-26 in EtOH-treated L-02 cells transfected with Rev-erbα shRNA (scale bars = 40 μm) (n ≥ 3). Bar represents the mean ± SEM. Significance *P < 0.05, **P < 0.01 vs. control group. ^#P < 0.05, ^##P < 0.01 vs. control shRNA group