Fig. 3

SR8278 (Rev-erbα antagonist) attenuates steatosis in the liver of EtOH-fed mice and EtOH-treated L-02 cells. Mice fed EtOH liquid were tail vein injection with SR8278 (2 mg/kg) for 3 days. A Representative image of liver and histological assessment of hepatic pathologic alterations by H&E and ORO staining in the liver of mice with or without SR8278 injection (scale bar = 100 μm or 20 μm). B–E The liver weight ratio to body of mice, serum ALT, TG level and TC levels in the liver of mice. F The immunohistochemistry staining of Pparα and Srebp1c in the liver of mice (scale bar = 100 μm or 20 μm) (n = 6). Bar represents the mean ± SEM. Significance *P < 0.05, **P < 0.01 vs. CD-fed group. ^#P < 0.05, ^##P < 0.01 vs. EtOH-fed group