Fig. 9

Disruption of Lrp4 β-gal synaptic expression by RKI-1447. TA muscles of adult lacZ/+ mice were injected with RKI-1447 or DMSO as control. a Representative images of β-gal, α-BTX (red) and SYN/NF (green). Scale bar, 0.2 mm. b Enlarged images of the box in (a). Scale bar, 50 µm. c–e No change in AChR cluster fragments (c), AChR area (d) and never coverage (e). f Decreased AChR clusters with β-gal activity. ***p = 0.0003. n > 4 mice per group, 30–40 NMJs per mice, unpaired t-test