Fig. 5
Genetical inhibition of ErbB4 kinase activity eliminates the effects of KD on inhibitory synaptic activity and seizure resistance. A Intraperitoneal injection of 1NM-PP1 (0.1 µg/g body weight) inhibited ErbB4 phosphorylation in T796G mice in 30 min and recovered in 60 min. B Quantitative analysis of data in A. One-way ANOVA, for ErbB4, F(2,6) = 0.4903, P = 0.635; for P-ErbB4/ErbB4, F(2,6) = 70.20, P < 0.0001. 0 min vs 30 min, P = 0.0006; 30 min vs 60 min, P = 0.0008. C Representative eIPSC traces from pyramidal neurons in CA1 region. Scale bars, 100 ms and 1000 pA. D Comparable amplitudes of eIPSCs in T796G mice fed with KD with those fed with CD after 1NM-PP1 injection. n = 13 neurons from 3 mice (CD + 1NM-PP1); n = 11 neurons from 3 mice (KD + 1NM-PP1). Two-way ANOVA, F(1,198) = 3.237, P = 0.0735. E No difference in the sIPSC frequencies between the two groups. Shown were representative sIPSC traces from pyramidal neurons in the CA1 region. Scale bars, 2 s and 50 pA. F Quantitative analysis of data in E. n = 13 neurons from 3 mice (CD + 1NM-PP1); n = 11 neurons from 3 mice (KD + 1NM-PP1). Student’s t test, t(22) = 0.2317, P = 0.8189. G Schematic of experimental design. After three weeks of diet feeding, T796G mice were injected with 1NM-PP1 20 min before KA infusion to induce seizures. H Comparable seizure score between the two groups. n = 5 T796G mice fed with CD, n = 6 T796G mice fed with KD. Student’s t test, t(9) = 0.02204, P = 0.9829