Fig. 6

Intravitreal injection of anti-Sema3A increased M2-like microglia and decreased RGCs apoptosis in the retina. a Representative confocal images and quantitative analysis showing immunofluorescent staining of CD206 (green), CD16/32 (red) and Iba1 (white) with Hoechst (blue) nuclear staining in retina from mice post-injury at 7 days with or without anti-Sema3A treatment. Scale bar = 100 μm. Quantitative analysis indicates a significant increase of CD206 with anti-Sema3A treatment and CD32 without anti-Sema3A at 7 days post-injury (Mean ± SEM, n = 5). Ratio of the number of double positive CD32/Iba1 cells vs. Iba1 positive cells (bar graph). Ratio of the number of double positive CD206/Iba1 cells vs. Iba1 positive cells (bar graph). b Representative confocal images of anti-Tuj1 and the quantitative analysis (n = 4) show reduction in RGCs (red arrows) and axons (white arrows) after ONC. anti-Sema3A alleviated RGCs apoptosis. Scale bar = 25 μm. c, d Expression of Pro-caspase3 and cleaved-caspase3 in the retina. Quantitative analysis indicates a significant increase of cleaved-caspase3 in the ONC group, which is reduced after anti-Sema3A injection (n = 6). β-actin was used as a loading control